matlab 2016r  (MathWorks Inc)

Journal: Stem Cells International

Article Title: Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells

doi: 10.1155/2016/1406304

Figure Lengend Snippet: The influence of fibronectin on the dynamic interaction of LO-EPC with control HAEC and HAEC with ischaemia reperfusion injury under 0.7 dyn/cm 2 shear stress. 3 × 10 4 HAEC were plated into Ibidi μ -Slide VI 0.4 Luer slides which had been precoated with 100 μ g/mL fibronectin. The data represent a mean ± SE of three experiments. LO-EPC adhesion to fibronectin was integrin dependent (b). LO-EPC binding to fibronectin was blocked by antibodies against integrin α 5 β 1, integrin α v β 1, and integrin α v β 3. The data represent the mean ± SE of three experiments. ∗∗ p < 0.01 compared to the control (no blocking antibodies).

Article Snippet: 1.5 mL of 4 × 10 5 LO-EPC was incubated with 10 μ g/mL anti-ITG α 5 β 1 (Millipore, UK), anti-ITG α V β 3 (Millipore, UK), and anti-ITG α v β 1 (Bioss, Antibodies-online.com) or no antibodies (control) for 30 minutes at 37°C with gentle rotation.

Techniques: Binding Assay, Blocking Assay